AFM Tip-Integrated Biosensors
National Institutes of Health (NIH);
Principle Investigator: Dr. B. Mizaikoff;
"Multifunctional Scanning Probes for Imaging of Cellular Signaling Processes"
Project Duration: 2003-2008
Selected Related Publications
Surface modification of AFM tip-integrated electrodes results in combined AFM-micro-/nanobiosensors, a novel technology enabling the detection of biologically relevant species during AFM imaging. Derived from strategies well established for amperometric biosensors, enzymes are immobilized onto the integrated electrode surface via self-assembled monolayers, covalent attachment, or polymer film entrapment. Imaging amperometric microbiosensors are gaining increasing interest, as they offer the advantageous combination of inherent selectivity provided by biological recognition elements with well-established transduction principles, along with obtaining topographical data correlated in space and time. Since the concept of AFM tip-integrated sensors is not limited to amperometric micro- and nanobiosensors, we are currently working on the integration of a wide variety of electrochemical sensors into scanning probe microscopy tip.
All studies are performed in a cross-disciplinary team of Co-PIs at Georgia Tech: Dr. C. Kranz (School of Chemistry and Biochemistry), Dr. A. Fedorov (School of Mechanical Engineering) and Emory University: Dr. D.C. Eaton (School of Physiology).
Selected Related Publications:
- J. Wiedemair, B. J. Duke, A. Kueng, D. Eaton, B. Mizaikoff, C. Kranz, Imaging of ATP release at live epithelial cells with AFM-tip integrated microbiosensors, Manuscript in preparation, 2006.
- A. Kueng, C. Kranz, A. Lugstein, E. Bertagnolli, B. Mizaikoff, AFM-tip-integrated amperometric microbiosensors: High-resolution imaging of membrane transport, Angew. Chem. Int. Ed., 44, 3419-3422, 2005.
- C. Kranz, A. Kueng, A. Lugstein, E. Bertagnolli, B. Mizaikoff, Mapping of enzyme activity by detection of enzymatic products during AFM imaging with integrated SECM-AFM probes, Ultramicroscopy, 100, 127-134, 2004.
